Directed evolution library creation : methods and protocols /

Directed protein evolution-a powerful technique for the discovery of new enzymes and therapeutic proteins-has matured to the point that it is now the centerpiece of enzyme engineering, where it has catalyzed the development of numerous experimental methods. In Directed Evolution Library Creation: Me...

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2003.
Description:1 online resource (x, 224 pages) : illustrations
Language:English
Series:Methods in molecular biology ; v. 231
Methods in molecular biology (Clifton, N.J.) ; v. 231.
Subject:Gene libraries -- Laboratory manuals.
Genetic engineering -- Laboratory manuals.
Molecular evolution -- Laboratory manuals.
Microbial mutation -- Laboratory manuals.
Combinatorial chemistry -- Laboratory manuals.
Genetic recombination -- Laboratory manuals.
Gene Library.
Combinatorial Chemistry Techniques.
Banques de gènes -- Manuels de laboratoire.
Génie génétique -- Manuels de laboratoire.
Évolution moléculaire -- Manuels de laboratoire.
Micro-organismes -- Mutation -- Manuels de laboratoire.
Chimie combinatoire -- Manuels de laboratoire.
Recombinaison génétique -- Manuels de laboratoire.
SCIENCE -- Life Sciences -- Molecular Biology.
Combinatorial chemistry.
Gene libraries.
Genetic engineering.
Genetic recombination.
Microbial mutation.
Molecular evolution.
Genetica.
Moleculaire biologie.
Genetische manipulatie.
Wetenschappelijke technieken.
Engenharia genética.
Biologia molecular.
Mutagênese.
Recombinação genética.
Fulltext.
Illustration.
Internet Resource.
Laboratory Manual.
Laboratory manuals.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11154630
Hidden Bibliographic Details
Other authors / contributors:Arnold, Frances Hamilton.
Georgiou, George.
ISBN:9781592593958
1588292851
9781588292858
159259395X
1280843233
9781280843235
9786610843237
6610843236
9781617374715
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:Directed protein evolution-a powerful technique for the discovery of new enzymes and therapeutic proteins-has matured to the point that it is now the centerpiece of enzyme engineering, where it has catalyzed the development of numerous experimental methods. In Directed Evolution Library Creation: Methods and Protocols, seasoned practitioners from many leading laboratories share their best experimental protocols for the generation of molecular diversity. Described in step-by-step detail to ensure experimental success, these protocols include readily reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologous recombination, and for constructing in vivo libraries in bacteria and yeast. In addition to the various protocols for creating libraries, this volume also describes ways to analyze libraries, particularly those made by recombination. An accompanying volume, Directed Enzyme Evolution: Screening and Selection Methods (ISBN: 1-58829-286-X), is devoted entirely to selection and screening methods that can be applied to the directed evolution of enzymes. Taken together, Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods capture for newcomers and more experienced investigators alike all the key methods for using directed protein evolution to better understand protein structure-function relationships, to discover new enzymes and therapeutic proteins, and to design new assays suitable for specific applications.
Other form:Print version: Directed evolution library creation. Totowa, N.J. : Humana Press, ©2003 1588292851 9781588292858
Standard no.:10.1385/159259395X.