Fluorescent and luminescent probes for biological activity : a practical guide to technology for quantitative real-time analysis /

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Bibliographic Details
Edition:2nd ed.
Imprint:San Diego, Calif. : Academic Press, ©1999.
Description:1 online resource (xxv, 647 pages) : illustrations (some color)
Language:English
Series:Biological techniques
Biological techniques series.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11153496
Hidden Bibliographic Details
Other authors / contributors:Mason, W. T.
ISBN:9780124478367
0124478360
9780080531779
0080531776
1281057002
9781281057006
9786611057008
6611057005
Notes:Includes bibliographical references and index.
Restrictions unspecified
Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010.
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212
English.
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Print version record.
Summary:The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes. The contents include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors, cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research. TOPICS COVERED: * Single and multiphoton confocal microscopy * Applications of green fluorescent protein and chemiluminescent reporters to gene expression studies * Applications of new optical probes for imaging proteins in gels * Probes and detection technologies for imaging membrane potential in live cells * Use of optical probes to detect microorganisms * Raman and confocal raman microspectroscopy * Fluorescence lifetime imaging microscopy * Digital CCD cameras and their application in biological microscopy.
Other form:Print version: Fluorescent and luminescent probes for biological activity. 2nd ed. San Diego, Calif. : Academic Press, ©1999 0124478360 9780124478367