Single molecule biology /

Single molecule biology /

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Bibliographic Details
Imprint:Amsterdam ; Boston : Elsevier/Academic, c2009.
Description:xxxv, 332 p. : ill. ; 25 cm.
Language:English
Subject:Molecular biology.
Einzelmolekülspektroskopie.
Freies Molekül.
Kontraktile Proteine.
Einzelmolekülspektroskopie.
Freies Molekül.
Kontraktile Proteine.
Molecular biology.
Aufsatzsammlung.
Aufsatzsammlung.
Format: Print Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/7694265
Hidden Bibliographic Details
Other authors / contributors:Knight, Alex. (Alexander Edward)
ISBN:0123742277 (hbk.)
9780123742278 (hbk.)
Notes:Includes bibliographical references and index.
Summary:"This book reveals how single molecule techniques have revolutionized our understanding of biology at the nanoscale - and it is the first book to be focused on the biology, rather than the techniques themselves. As such, it is targeted at biologists and a broad interdisciplinary readership. Each chapter in this multicontributed book is written by an expert in the field, covering a key biological system studied by single molecule methods, and exploring what has been learnt from this new approach. Systems examined include molecular motors (kinesin, dynein, myosin, and the bacterial flagellar motor); nucleic acids (chromatin, DNA-based enzymes, and the ribosome); membranes and ion channels; and a look at applications to microarray technology."--BOOK JACKET.
Table of Contents:
  • Preface
  • Acknowledgments
  • Microarray Surfaces: There is Plenty to Groom at the Bottom
  • Applications: Learning from Singles
  • DNA Methylation Analysis
  • DNA Fragment Sizing
  • DNA (Combing and) Mapping
  • Single Molecule Sequencing
  • Conclusions
  • Acknowledgments
  • References
  • Appendix
  • References
  • Index
  • Chapter 1. Single Molecule Studies of Myosins
  • Introduction
  • Motility Assays
  • Molecular Mechanics
  • Technologies
  • The Myosin Working Stroke
  • Force and Stiffness
  • Kinetics
  • Acknowledgments
  • Conclusions
  • Acknowledgments
  • References
  • Chapter 2. Single Molecule Experiments and the Kinesin Motor Protein Superfamily: Walking Hand in Hand
  • Introduction
  • Overall Mechanical Parameters of Kinesins
  • Advanced Mechanochemistry of Kinesin-1
  • Lattice Diffusion as an Additional Motility Mode
  • Regulation of Kinesin Motors
  • Final Thoughts
  • List of Contributors
  • Acknowledgments
  • References
  • Chapter 3. Force-Generating Mechanisms of Dynein Revealed through Single molecule Studies
  • Introduction
  • Molecular Organization of Dynein
  • Mechanism of Force Generation by Dyneins
  • Mechanical Properties of Dyneins Studied by Single molecule Methods
  • Dyneins in Axonemes
  • Perspectives: From Single Molecules to Ensembles
  • Acknowledgments
  • Introduction: The "Single Molecule" Paradigm
  • References
  • Chapter 4. The Bacterial Flagellar Motor
  • Introduction
  • Structure
  • Function
  • Outlook
  • References
  • Chapter 5. Single Molecule Studies of Chromatin Structure and Dynamics
  • Introduction
  • Sperm Chromatin
  • The "Single Molecule" Paradigm
  • Spermiogenesis
  • Previous Studies of Toroid Structure
  • Single molecule Experiments
  • The Protamines P1 and P2
  • The Transition Proteins TP1 and TP2
  • Shaping of the Sperm Head and the Role of the Manchette
  • Posttranscriptional Modifications of Sperm Nuclear Proteins
  • Conclusions: Sperm Chromatin
  • Somatic Chromatin
  • Conclusion
  • Life as a Molecule
  • Acknowledgments
  • References
  • Chapter 6. Single Molecule Studies of Nucleic Acid Enzymes
  • Introduction
  • Methods
  • Single molecule Studies of Nucleic Acid Enzymes
  • Conclusion
  • References
  • Chapter 7. Single Molecule Studies of Prokaryotic Translation
  • Introduction
  • Single Molecule Techniques
  • Directly Observing Translation
  • Translation at Atomic Resolution
  • Single molecule Translation
  • Conclusions
  • References
  • Chapter 8. Single Ion Channels
  • Comparison of Fluorescence Methods with Single-Channel Recording
  • How to Get Information about Mechanisms from Single molecule Measurements
  • Some Recent Results from Single-Channel Recording
  • Conclusions
  • Overview of Single Molecule Biology
  • References
  • Chapter 9. Single Molecule Fluorescence in Membrane Biology
  • The Structure and Function of Cellular Membranes
  • Why Apply Single molecule Fluorescence to Membranes?
  • Fluorescent Labels and Artificial Cell Membranes
  • Single molecule Fluorescence Techniques
  • The Role of SMF in Studying Membranes and Membrane Proteins
  • Probing the Structure and Behavior of Cell Membranes
  • Membrane-Cytoskeleton Interactions
  • Lipid Rafts
  • Conclusions
  • Understanding the Dynamics and Interactions of Membrane Proteins
  • Signalling
  • Membrane Transport
  • Vesicle Tracking, Docking, and Fusion
  • Conclusions
  • References
  • Chapter 10. Single Molecule Microarray Analysis
  • Introduction
  • Speed and Sensitivity: Mutually Exclusive Demands?
  • Microarrays: Attempting a Definition
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