The eukaryotic RNA exosome : methods and protocols /

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Bibliographic Details
Imprint:New York : Humana Press, [2020]
Description:1 online resource (1 PDF file (xiii, 517 pages)) : illustrations (some color)
Language:English
Series:Methods in molecular biology, 1940-6029 ; volume 2062
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 2062.
Springer protocols (Series)
Subject:Eukaryotic cells.
RNA.
Exosome Multienzyme Ribonuclease Complex.
RNA -- metabolism.
Eukaryotic Cells.
Eukaryotic cells.
RNA.
Laboratory Manual.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11998162
Hidden Bibliographic Details
Other authors / contributors:LaCava, John, editor.
Vaňáčová, Štěpánka, editor.
ISBN:9781493998227
1493998226
9781493998210
Notes:Includes bibliographical references and index.
Online resource; title from PDF title page (SpringerLink, viewed December 17, 2019).
Summary:This volume provides a cross-section of RNA exosome research protocols, applied to a diversity of model organisms. Chapters guide readers through methods that e.g. delineate eukaryotic exosomes' origins in prokaryotes, probe its RNA substrates, adapter complexes, and macromolecular interaction of networks, and establish critical structure-function relationships. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. This book begins with an introduction to RNA exosome biomedical relevance (Chapter 1) and then steps back to examine the origins of exosome activity in bacteria (Chapters 2-3) and archaea (Chapter 4). From there, methods of studying exosome RNA substrates are covered (Chapters 5-10), followed by the study of exosome adapter complexes and the broader interaction networks that impart selectivity to pathways the exosome participates in (Chapters 11-16). Finally, structural and mechanistic biochemical studies are covered, with emphasis on methods that use recombinant expression and exogenous reconstitution (Chapters 17-24).
Standard no.:10.1007/978-1-4939-9822-7