Microbial transposon mutagenesis : protocols and applications /

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Bibliographic Details
Imprint:New York, NY : Humana Press, 2019.
Description:1 online resource (xi, 183 p.) : ill. (some col.).
Series:Methods in molecular biology, 1940-3029 ; volume 2016
Methods in molecular biology (Clifton, N.J.) ; v. 2016.
Subject:Microbial genetics.
Microbial genomics.
Microbial genetics.
Microbial genomics.
Electronic books
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11913280
Hidden Bibliographic Details
Other authors / contributors:Ricke, Steven C., 1957- editor.
Park, Si Hong, editor.
Davis, Morgan L., editor.
Digital file characteristics:text file PDF
Summary:This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism. The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as Salmonella to Bifidobacterium, a microorganism considered beneficial to humans and animals. The final section addresses more general protocols including plasmid transfer and bioinformatic tools as well as novel applications of transposon methodologies such as transposon-aided capture of antibiotic resistant plasmids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Transposon Mutagenesis: Protocols and Applications serves as a valuable reference for scientists seeking to apply transposon mutagenesis to microbial genetic analyses and functionality.
Other form:Printed edition 9781493995691
Printed edition 9781493995714
Printed edition 9781493995721
Standard no.:10.1007/978-1-4939-9570-7