Surface enhanced raman spectroscopy for biophysical applications : using plasmonic nanoparticle assemblies /

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Bibliographic Details
Author / Creator:Fasolato, Claudia, author.
Imprint:Cham, Switzerland : Springer, 2018.
Description:1 online resource (xviii, 155 pages) : illustrations (some color)
Series:Springer theses, 2190-5053
Springer theses,
Subject:Raman spectroscopy.
Raman effect, Surface enhanced.
Raman effect, Surface enhanced.
Raman spectroscopy.
Electronic books.
Format: E-Resource Book
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Digital file characteristics:text file PDF
Notes:"Doctoral thesis accepted by the Sapienze University of Rome, Rome, Italy."
Online resource; title from PDF title page (SpringerLink, viewed December 14, 2018).
Summary:The book explores the phenomenon of surface-enhanced Raman scattering (SERS), the huge amplification of Raman signal from molecules in the proximity of a metallic nanostructured surface, allowing readers to gain an in-depth understanding of the mechanisms affecting the spectroscopic response of SERS-active systems for effective applications. SERS spectroscopy is an ultrasensitive analytical technique with great potential for applications in the field of biophysics and nanomedicine. As examples, the author presents the design of nanocolloid-based SERS-active substrates for molecular sensing and of a folate-based SERS-active nanosensor capable of selectively interacting with cancer cells, enabling cancer diagnostics and therapy at the single-cell level. The author also suggests novel paths for the systematization of the SERS nanosystem design and experimental protocols to maximize sensitivity and reproducibility, which is essential when real-world biomedical applications are the goal of the study. With a combined approach, both fundamental and applied, and a detailed analysis of the state of the art, this book provides a valuable overview both for students new to SERS spectroscopy and for experts in the field.
Other form:Printed edition: 9783030035556
Printed edition: 9783030035570
Standard no.:10.1007/978-3-030-03556-3
Table of Contents:
  • Intro; Supervisor's Foreword; Abstract; Parts of this thesis have been published in the following articles; Acknowledgements; Contents; 1 Introduction; References; 2 Traditional Raman and SERS: Fundamentals and State of the Art; 2.1 Optical Vibrational Spectroscopy of Molecules; 2.1.1 Theory of Raman Scattering; 2.2 Surface Enhanced Raman Scattering; 2.2.1 Electromagnetic Mechanism; 2.2.2 Chemical Mechanism; 2.3 Current Trends in Surface Enhanced Raman Scattering; 2.3.1 Nanoparticle-Based SERS Substrates; 2.3.2 SERS Applications in Nanomedicine
  • 2.3.3 Non Metallic Substrates: SERS from Semiconductor NanostructuresReferences; 3 Investigation on Nanoparticles and Their Molecular Functionalization; 3.1 Model Functionalization with 4ATP Linker; 3.1.1 Preparation and Control Characterization; 3.1.2 SERS Versus Raman Spectrum; 3.2 Further Conjugation: Folate and Antifolates; 3.2.1 Nanovector Functionalization: The Case of Folate; 3.2.2 SERS Signature of Folate/Antifolate Nanovectors; 3.3 Experimental Details; References; 4 Nanoparticle-Based SERS Substrates for Molecular Sensing Applications
  • 4.1 Self-assembled Mesoscopic Silver Nanoparticle Clusters4.1.1 Coupled AFM and Raman Analysis; 4.1.2 Dimensional Scale Effect on SERS Efficiency; 4.2 FDTD Simulation: Theoretical Predictions; 4.2.1 The FDTD Software; 4.2.2 Results on Silver and Gold Np Multilayers; 4.3 Electron Beam Lithography Driven Self-assembly; 4.3.1 Nanoparticle Cluster Array Preparation; 4.3.2 Analysis of the Patterned Arrays; 4.3.3 Analysis of the Ordered Nanoparticle Cluster Arrays; 4.3.4 SERS Reproducibility; References; 5 SERS-Active Nanovectors for Single-Cell Cancer Screening and Theranostics
  • 5.1 Early Cancer Diagnostics and Theranostics5.2 Folate-Based SERS Diagnostics; 5.2.1 Idea of the Experiment; 5.2.2 Cell Treatment and Viability Tests; 5.2.3 Raman and SERS Imaging on Treated Cells; 5.2.4 SEM Imaging on Treated Cells; 5.2.5 SERS Screening and Results; 5.3 From Selectivity to Theranostics: Antifolate Nanovectors; 5.3.1 Preliminary Cytotoxicity Results; 5.3.2 Enhanced Toxicity of Nanostructured Drugs; References; 6 Conclusions; A SERS Application in Cultural Heritage; A.1 HPTLC-SERS Investigation of Madder Lake Ammonia Extract; A.1.1 HPTLC-SERS Analytical Method
  • A.1.2 Experimental ResultsA. 2 A Multianalytical Study Unravels Purple Dyeing Processes: The Case of a Historical Dress from the 19th Century; A.2.1 Introduction; A.2.2 Sicilian Noblewoman Dress: Microscopy, Sampling, Experimental Details; A.2.3 Results and Discussion; A.2.4 Conclusions; Curriculum Vitae