Aqueous two-phase systems for bioprocess development for the recovery of biological products /

Aqueous two-phase systems for bioprocess development for the recovery of biological products /

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Bibliographic Details
Imprint:Cham : Springer, 2017.
Description:1 online resource
Language:English
Series:Food engineering series, 1571-0297 ; v. 1
Food engineering series ; v. 1.
Subject:
Phase partition.
Biochemical engineering.
Biochemical engineering.
Phase partition.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11323155
Hidden Bibliographic Details
Other authors / contributors:Rito Palomares, Marco Antonio.
Benavides, Jorge.
ISBN:9783319593098
3319593099
9783319593081
3319593080
Digital file characteristics:text file PDF
Notes:Includes index.
Includes bibliographical references at the end of each chapters and index.
Print version record.
Summary:This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area. Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.
Other form:Print version: Aqueous two-phase systems for bioprocess development for the recovery of biological products. Cham : Springer, 2017 9783319593081 3319593080
Standard no.:10.1007/978-3-319-59309-8
Table of Contents:
  • Preface; Contents; Contributors; 1: General Concepts and Definitions of Aqueous Two-Phase Systems; 1.1 Introduction; 1.2 Binodal Curve, Tie-Line Length, and Volume Ratio; 1.3 General Classification of ATPS; 1.3.1 Polymer-Polymer Systems; 1.3.2 Polymer-Salt Systems; 1.3.3 Alcohol-Salt Systems; 1.3.4 Micellar Systems; 1.3.5 Ionic Liquid-Based Systems; 1.4 Definitions of Parameters of ATPS: Partition Coefficient, Selectivity, Recovery Yield, and Purity; 1.4.1 Partition Coefficient; 1.4.2 Selectivity; 1.4.3 Recovery Yield; 1.4.4 Purity.
  • 1.5 Factors that Affect Partitioning in ATPS: Molecular Weight, Electrochemical Interactions and pH, Hydrophobicity, and Affinity1.5.1 Molecular Weight or Size; 1.5.2 Electrochemical Interactions and pH; 1.5.3 Hydrophobicity; 1.5.4 Affinity; 1.6 Concluding Remarks; References; 2: Characterization of Aqueous Two-Phase Systems and Their Potential New Applications; 2.1 Introduction; 2.2 Aqueous Two-Phase System Characterization; 2.3 Solute Partition Characterization; 2.4 Novel Operational Strategies and Potential New Applications for Aqueous Two-Phase Systems; 2.5 Concluding Remarks.
  • 3.3.3 Recovery of Invertase from Wasted Brewer's Yeast3.4 ATPS Strategies for the Recovery of PEGylated Proteins; 3.5 Protein Refolding Explorations in ATPS; 3.6 Concluding Remarks; References; 4: Aqueous Two-Phase Systems for the Recovery of Bioparticles; 4.1 Introduction; 4.2 Partitioning of Whole Cells, Stem Cells, and Cellular Components in ATPS; 4.2.1 Blood Components; 4.2.2 Stem Cells; 4.2.3 Other Cell Types; 4.3 Cell Components; 4.4 Partition of Viral Particles: Toward New Therapeutic Strategies; 4.5 Nucleic Acids Recovery in ATPS: Current Innovations.
  • 4.6 Macroparticle Recovery with ATPS: Challenges and Future Trends4.7 Concluding Remarks; References; 5: Aqueous Two-Phase System Strategies for the Recovery and Partial Purification of Bioactive Low Molecular Weight Compounds; 5.1 Introduction; 5.2 Evolution of the Use of Aqueous Two-Phase Systems for the Recovery and Partial Purification of Low Molecular Weight Compounds; 5.3 Selected Studies on the Extraction, Recovery, and Partial Purification of Low Molecular Weight Compounds Using Aqueous Two-Phase Systems; 5.3.1 Polymer-Polymer and Polymer-Salt ATPS; 5.3.2 Alcohol-Salt ATPS.

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