Optogenetics.
Saved in:
| Author / Creator: | Hegemann, Peter. |
|---|---|
| Imprint: | Berlin : De Gruyter, 2013. |
| Description: | 1 online resource (240 pages) |
| Language: | English |
| Series: | Dahlem Workshop Reports Dahlem workshop reports. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11204879 |
Table of Contents:
- List of contributing authors; Introduction; 1 The biophysics and engineering of signaling photoreceptors; 1.1 Photoreceptors; 1.1.1 Novel photoreceptors; 1.1.2 Biophysics of photoreceptors and signal transduction; 1.2 Engineering of photoreceptors; 1.2.1 Approaches to designing light-regulated biological processes; 1.3 Case study
- transcriptional control in cells by light; 1.4 Conclusion; Acknowledgements; References; 2 Current challenges in optogenetics; 2.1 Introduction; 2.2 Background: current functionality of tools.
- 2.3 Unsolved problems and open questions: technology from cell biology, optics, and behavior2.4 Unsolved problems and open questions: genomics and biophysics; 2.5 Conclusion; References; 3 Challenges and opportunities for optochemical genetics; 3.1 Introduction; 3.2 Photosensitizing receptors; 3.3 PCL and PTL development and applications; 3.4 Advantages and disadvantages of PCLs and PTLs; 3.5 Conclusion; References; 4 Optogenetic imaging of neural circuit dynamics using voltage-sensitive fluorescent proteins: potential, challenges and perspectives; 4.1 Introduction; 4.2 The biological problem.
- 4.3 The large scale challenge of circuit neurosciences4.4 The current approach to the large-scale integration problem; 4.5 Large-scale recordings of neuronal activities using optogenetic approaches; 4.6 Genetically encoded voltage indicators: state of development and application; 4.7 Unsolved methodological / technical challenges; References; 5 Why optogenetic "control" is not (yet) control; Acknowledgments; References; 6 Optogenetic actuation, inhibition, modulation and readout for neuronal networks generating behavior in the nematode Caenorhabditis elegans.
- 6.1 Introduction
- the nematode as a genetic model in systems neurosciencesystems neuroscience6.2 Imaging of neural activity in the nematode; 6.2.1 Genetically encoded Ca2+ indicators (GECIs); 6.2.2 Imaging populations of neurons in immobilized animals; 6.2.3 Imaging neural activity in freely moving animals; 6.2.4 Other genetically encoded indicators of neuronal function; 6.3 Optogenetic tools established in the nematode; 6.3.1 Channelrhodopsin (ChR2) and ChR variants with different functional properties for photodepolarization.
- 6.3.2 Halorhodopsin and light-triggered proton pumps for photohyperpolarization6.3.3 Photoactivated Adenylyl Cyclase (PAC) for phototriggered cAMPdependent effects that facilitate neuronal transmission; 6.3.4 Other optogenetic approaches; 6.3.5 Stimulation of single neurons by optogenetics in freely behaving C. elegans; 6.4 Examples for optogenetic applications in C. elegans; 6.4.1 Optical control of synaptic transmission at the neuromuscular junction and between neurons; 6.4.2 Optical control of neural network activity in the generation of behavior; 6.5 Future challenges.