RNA turnover in eukaryotes : analysis of specialized and quality control RNA decay pathways /
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| Imprint: | San Diego, Calif. : Academic, 2008. |
|---|---|
| Description: | 1 online resource (xliv, 417 pages, [1] leaf of plates) : illustrations (some color). |
| Language: | English |
| Series: | Methods in enzymology, 0076-6879 ; v. 449 Methods in enzymology ; volume 449. |
| Subject: | Protista. RNA. RNA Precursors. RNA Stability -- physiology. Eukaryotic Cells -- metabolism. RNA, Messenger -- metabolism. mRNA Cleavage and Polyadenylation Factors. SCIENCE -- Life Sciences -- Biology. SCIENCE -- Life Sciences -- Microbiology. Protista. RNA. Laboratory Manuals. Electronic books. |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11195230 |
Table of Contents:
- Front Cover; RNA Turnover in Eukaryotes: Analysis of Specialized and Quality Control RNA Decay Pathways; Copyright Page; Contents; Contributors; Preface; Volumes in Series; Section I: Analysis of Specialized mRNA Decay Pathways; Chapter 1: Methods to Study No-Go mRNA Decay in Saccharomyces Cerevisiae; 1. Introduction; 2. Design and Construction of an NGD Substrate mRNA; 4. Conclusion; References; Chapter 2: Cell-Cycle Regulation of Histone mRNA Degradation in Mammalian Cells: Role of Translation and Oligouridylation; 1. Introduction.
- 2. Use of the Iron Response Protein to Study the Role of Translation in Histone mRNA Degradation3. Expression of a Dominant Negative Stem-Loop Binding Protein; 5. Oligo(dA) RT-PCR to Visualize Oligo(U) Tails on Histone mRNA following Inhibition of DNA Synthesis or at the End of S Phase; Chapter 3: Assays of Adenylate Uridylate-Rich Element-Mediated mRNA Decay in Cells; 1. Introduction; 2. Reporter Gene System; 6. Quantitation of mRNA Levels; Acknowledgments; References; Chapter 4: Evaluating the Control of mRNA Decay in Fission Yeast; 1. Introduction.
- 3. Systems for Studying TZF Protein-Mediated mRNA Decay5. Use of the nmt Expression System to Evaluate zfs1-Mediated mRNA Decay; 6. Northern Blot Analysis and Transcript Quantitation; Acknowledgments; References; Chapter 5: In Vivo Analysis of the Decay of Transcripts Generated by Cytoplasmic RNA Viruses; 1. Introduction; 3. Viral RNA Decay Systems; 5. Analysis of the 3prime End of Viral RNA; 6. Isolation of Small RNAs; Acknowledgments; References; Section II: Nonsense-Mediated MRNA Decay (How Do You Study NMD; What Defines An NMD Target).
- Chapter 6: Qualitative and Quantitative Assessment of the Activity of the Yeast Nonsense-Mediated mRNA Decay Pathway1. Introduction; 2. Methods and Discussion; 3. Summary; Acknowledgment; References; Chapter 7: Nonsense-Mediated mRNA Decay in Caenorhabditis Elegans; 1. Introduction; 3. Protocol for a Genome-Wide RNAi-Based NMD Screen; 4. Protocol: Genetic Screen for Novel NMD Factors; References; Chapter 8: In Vivo Analysis of Plant Nonsense-Mediated mRNA Decay; 1. Introduction.
- 5. Experiment 1 (Analysis of Endogeneous NMD Target: The Fate of At3g63340 Splicing Variants in Arabidopsis Thaliana)6. Experiment 2 (Recognition of Termination Codon Contexts as NMD Targets in Nicotiana Benthamiana); References; Chapter 9: Studying Nonsense-Mediated mRNA Decay in Mammalian Cells; 1. Introduction; 2. Criteria for Nonsense-Mediated mRNA Decay in Mammalian Cells; 3. Methods Used to Study NMD in Cultured Mammalian Cells; Acknowledgments; Section III: Analysis of Nuclear MRNA Decay and Non-MRNA Decay; Chapter 10: Estimating Nuclear mRNA Decay in Saccharomyces Cerevisiae.