Recombinant gene expression protocols /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©1997.
Description:1 online resource (xx, 521 pages) : illustrations (some color)
Language:English
Series:Methods in molecular biology ; v. 62
Methods in molecular biology (Clifton, N.J.) ; v. 62.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11179015
Hidden Bibliographic Details
Other authors / contributors:Tuan, Rocky S.
ISBN:0896034801
9780896034808
9781592595488
1592595480
0896033333
9780896033337
9780896034808
Notes:Includes bibliographical references and index.
Print version record.
Summary:Annotation Expert international research workers offer the most up-to-date and comprehensive collection of techniques available for expressing one or more genes of interest. The authoritative contributors describe in depth the vectors used for expression in bacteria, yeast, viruses, nonmammalian higher eukaryotes, mammals, and plants, as well as in transgenic animals. They also provide invaluable detailed information on how to introduce the recombinant gene construct into the appropriate expression host. When combined with a companion volume by the same editor, Recombinant Proteins: Detection and Isolation Protocols, both volumes guide the research worker through the entire journey of recombinant gene expression.
Other form:Print version: Recombinant gene expression protocols. Totowa, N.J. : Humana Press, ©1997 0896033333

MARC

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245 0 0 |a Recombinant gene expression protocols /  |c edited by Rocky S. Tuan. 
260 |a Totowa, N.J. :  |b Humana Press,  |c ©1997. 
300 |a 1 online resource (xx, 521 pages) :  |b illustrations (some color) 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in molecular biology ;  |v v. 62 
504 |a Includes bibliographical references and index. 
505 0 |a Recombinant gene expression protocols : overview of experimental strategies for the expression of recombinant genes / Rocky S. Tuan -- Designing expression plasmid vectors in E. coli / Paulina Balbás -- Design of bacterial hosts for lac-based expression vectors / Herbert P. Schweizer, RoxAnn R. Karkhoff-Schweizer -- Bacteriophage [lambda]-based expression vectors / Alan C. Christensen -- Strategies for gene fusions / Stefan Ståhl, Per-Åke Nygren, Mathias Uhlén -- Application of the E. coli trp promoter / Daniel G. Yansura, Steven H. Bass -- Expression of proteins in E. coli utilizing a dual promoter-based vector : pLACT7 / George A. Garcia, Shaorong Chong -- Expression and secretion of proteins in E. coli / Ophry Pines, Masayori Inouye -- Synthetic two-cistron expression system / Brigitte E. Schoner -- Expression of cDNA-encoded proteins by cell-free transcription and translation / Chistopher Coleclough -- Yeast plasmids / Keshav K. Singh, Jack A. Heinemann -- Inducible expression cassettes in yeast. GAL4 / Lawrence M. Mylin, James E. Hopper -- Inducible expression cassettes in yeast. ADH2 / Virginia L. Price -- Constitutive expression vectors : PGK / Ian R. Graham, Alistair Chambers -- Design and construction of recombinant vaccinia viruses / Christopher C. Broder, Patricia L. Earl -- A simplified method of screening for isolation of recombinant vaccinia virus / Haifeng Chen, R. Padmanabhan -- Design of retroviral expression vectors / Kenine E. Comstock, Nathaniel F. Watson, John C. Olsen -- Use of defective herpes-derived plasmid vectors / Filip Lim, Dean Hartley, Philip Starr, Song Song, Phung Lang, Linda Yu, Yaming Wang, Alfred I. Geller -- Use of baculovirus expression vectors / David R. O'Reilly -- Expression of exogenous genes in Xenopus oocytes, eggs, and embryos / Koichiro Shiokawa, Chie Koga, Yuzuru Ito, Mikihito Shibata -- Analysis of heterologous gene expression in Xenopus blastomeres / Sally A. Moody -- Overview of vector design for mammalian gene expression / Randal J. Kaufman -- Experimental strategies in efficient transfection of mammalian cells. Calcium phosphate and DEAE-Dextran / Gregory S. Pari, Wayne A. Keown -- Experimental strategies in efficient transfection of mammalian cells. Electroporation / Donald C. Chang -- Highly effective delivery of foreign DNA to adherent cells via Polybrene/DMSO-assisted gene transfer / Rémy A. Aubin, Michael Weinfeld, Marzieh Taghavi, Razmik Mirzayans, Malcolm C. Paterson -- Selection of transfected cells : magnetic affinity cell sorting / Raji Padmanabhan, Snorri S. Thorgeirsson, R. Padmanabhan -- Selection of transfected cells and coamplification of transfected genes / Susan E. Kane -- Optimization of growth, viability, and specific productivity for expression of recombinant proteins in mammalian cells / Jennie P. Mather, Alison Moore, Robert Shawley -- Promoters to express cloned genes uniformly in Drosophila / Jean-Paul Vincent, Charles Girdham -- Application of micromechanical piercing structures for genetic transformation of nematodes / Sarwar Hashmi, Randy Gaugler -- Strategies in generating transgenic mammals / Olena Jacenko -- Use of gonadal primordial germ cells (PGCs) as tools for gene transfer in chickens / Nathalie Allioli, Gérard Verdier, Catherine Legras -- Strategies for the production of transgenic chickens / Robert J. Etches, Ann M. Verrinder Gibbins -- Plant cell transfection by electroporation / William B. Terzaghi, Anthony R. Cashmore -- Transformation and foreign gene expression in plants mediated by microprojectile bombardment / Henry Daniell -- Transformation of the cereals using agrobacterium / Jean Gould -- Transformation of microalgae using silicon carbide whiskers / Terri G. Dunahay, Sally A. Adler, Jonathan W. Jarvik. 
588 0 |a Print version record. 
520 8 |a Annotation  |b Expert international research workers offer the most up-to-date and comprehensive collection of techniques available for expressing one or more genes of interest. The authoritative contributors describe in depth the vectors used for expression in bacteria, yeast, viruses, nonmammalian higher eukaryotes, mammals, and plants, as well as in transgenic animals. They also provide invaluable detailed information on how to introduce the recombinant gene construct into the appropriate expression host. When combined with a companion volume by the same editor, Recombinant Proteins: Detection and Isolation Protocols, both volumes guide the research worker through the entire journey of recombinant gene expression. 
650 0 |a Genetic recombination  |v Laboratory manuals. 
650 1 2 |a Recombination, Genetic. 
650 2 2 |a Gene Expression. 
650 2 2 |a Genetic Engineering. 
650 7 |a Genetic recombination.  |2 fast  |0 (OCoLC)fst00940079 
653 0 0 |a genetische modificatie 
653 0 0 |a genetic engineering 
653 0 0 |a moleculaire genetica 
653 0 0 |a molecular genetics 
653 0 0 |a recombinant dna 
653 1 0 |a Molecular Genetics 
653 1 0 |a Moleculaire genetica 
655 2 |a Laboratory Manual. 
655 4 |a Fulltext. 
655 4 |a Internet Resource. 
655 4 |a Laboratory Manuals. 
655 7 |a Laboratory manuals.  |2 fast  |0 (OCoLC)fst01920776 
700 1 |a Tuan, Rocky S.  |0 http://id.loc.gov/authorities/names/n96109423 
776 0 8 |i Print version:  |t Recombinant gene expression protocols.  |d Totowa, N.J. : Humana Press, ©1997  |z 0896033333  |w (DLC) 96049600  |w (OCoLC)35928451 
830 0 |a Methods in molecular biology (Clifton, N.J.) ;  |v v. 62. 
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