Environmental genomics /

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Bibliographic Details
Imprint:	Totowa, N.J : Humana Press, ©2008.
Description:	1 online resource (xi, 364 pages) : illustrations
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; 410 Methods in molecular biology (Clifton, N.J.) ; v. 410.
Subject:	Ecological genetics. Microbial ecology. Microbial diversity. Microbiology -- Cultures and culture media. Environmental Monitoring -- methods. Gene Expression Profiling -- methods. Mutagenicity Tests -- methods. Ecological genetics. Microbial diversity. Microbial ecology. Microbiology -- Cultures and culture media. Laboratory Manual.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11170770

Hidden Bibliographic Details
Other authors / contributors:	Martin, C. Cristofre.
ISBN:	9781597455480 1597455482 1588297772 9781588297778 1588297772 9781588297778
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	Environmental genomics seeks to predict how an organism or organisms will respond, at the genetic level, to changes in their external environment. These genome responses are diverse and, as a result, environmental genomics must integrate molecular biology, physiology, toxicology, ecology, systems biology, epidemiology and population genetics into an interdisciplinary research program. Environmental Genomics serves as a manual for an environmental scientist who wishes to embrace genomics in an effort to answer environmental questions. The volume is divided into the sections: gene expression profiling, whole genome and chromosome mutation detection, and methods to assay genome diversity and polymorphisms within a particular environment. The studies presented will reduce the uncertainties associated with environmental risk assessment and provide a systematic framework for determining environmental impact and ensuring human health and the sustainability of natural populations. Environmental Genomics should aid researchers looking to reduce the uncertainties associated with environmental risk assessment and provide a systematic framework for determining environmental impact and ensuring human health and the sustainability of natural populations.
Other form:	Print version: Environmental genomics. Totowa, N.J : Humana Press, ©2008 1588297772
Standard no.:	10.1007/978-1-59745-548-0 9781588297778

Table of Contents:

Preface
Contributors
pt. 1. Gene expression profiling
1. High-throughput whole mount in situ hybridization of zebrafish embryos for analysis of tissue-specific gene expression changes after environmental perturbation / Louise E. Coverdale, Lindsay E. Burton, and C. Cristofre Martin
2. Fluorescent RNA arbitrarily primed polymerase chain reaction / Doug Crump [and others]
3. Isolation of O₃-response genes from Arabidopsis thaliana using cDNA macroarray / Masanori Tamaoki
4. Use of cDNA macroarrays and gene profiling for detection of effects of environmental toxicants / Jason L. Blum [and others]
5. Constructing and screening a cDNA library / Kevin Larade and Kenneth B. Storey
6. Comparative molecular physiological genomics / Sean F. Eddy and Kenneth B. Storey
7. Proteomic analysis of neuroendocrine peptidergic system disruption using the AtT20 pituitary cell line as a model / Fumin Dong [and others]
8. Proteomics-based method for risk assessment of peroxisome proliferating pollutants in the marine environment / Susana Cristobal
9. Environmental metabolomics using ¹H-NMR spectroscopy / Mark R. Viant
pt. 2. Detection of whole genome mutation
10. Restriction landmark genome scanning for the detection of mutations / Jun-ichi Asakawa
11. Use of the comet assay in environmental toxicology / Loren D. Knopper and James P. McNamee
12. The micronucleus assay determination of chromosomal level DNA damage / Michael Fenech
13. Fluorescence in situ hybridization for the detection of chromosome aberrations and aneuploidy induced by environmental toxicants / Francesca Pacchierotti and Antonella Sgura
14. Laboratory methods for the detection of chromosomal structural aberrations in human and mouse sperm by fluorescence in situ hybridization / Francesco Marchetti, Debby Cabreros, and Andrew J. Wyrobek
pt. 3. Determination of species diversity
15. Assembling DNA barcodes / Jeremy R. deWaard [and others]
16. Application of suppressive subtractive hybridization to uncover the metagenomic diversity of environmental samples / Elizabeth A. Galbraith, Dionysios A. Antonopoulos, and Bryan A. White
17. 16S rRNA targeted DGGE fingerprinting of microbial communities / Vesela A. Tzeneva [and others]
18. An emulsion polymerase chain reaction-based method for molecular haplotyping / James G. Wetmur and Jia Chen
Index.

Environmental genomics /

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