Environmental microbiology : methods and protocols /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2004.
Description:1 online resource (xiv, 423 pages) : illustrations
Series:Methods in biotechnology ; 16
Methods in biotechnology ; 16.
Subject:Microbial ecology.
Environmental Microbiology.
Bacteria -- isolation & purification.
Microbiological Techniques.
SCIENCE -- Life Sciences -- Biology.
SCIENCE -- Life Sciences -- Microbiology.
Microbial ecology.
Electronic books.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11130166
Hidden Bibliographic Details
Other authors / contributors:Spencer, J. F. T.
Ragout de Spencer, Alicia L.
Notes:Includes bibliographical references and index.
Print version record.
Summary:Understanding the relationship between a microorganism and its environment is essential to the successful manipulation of industrial, biochemical, and medical processes. In Environmental Microbiology: Methods and Protocols, highly practiced experimentalists who often have perfected the methods they write about describe readily reproducible techniques for determining most of the important factors governing microorganisms and their habitats. Presented in step-by-step detail, these cutting-edge methods range from those for the study of marine organisms, to those for investigating microorganisms occurring in groundwater, to the biodiversity found in remote environments. The protocols for studying fermented milks are significant for investigators concerned with milk as an item of food for infants, small children, and even adults. Additional methods for the recovery and determination of nucleic acids and other compounds affecting, and affected by, microorganisms, are provided for certain enzymes produced by plant pathogens and for obtaining microbial species tolerant of such inhibitors as heavy metals. Review articles discuss the endophytic bacterium Bacillus mojavensis, the engineering of bacteria to enhance their ability to carry out bioremediation of aromatic compounds, and the use of chemical shift reagents and Na-NMR to study sodium gradients in microorganisms. The protocols follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Environmental Microbiology: Methods and Protocols offers microbiological researchers a powerful set of techniques for investigating and understanding microorganisms in their native environments
Other form:Print version: Environmental microbiology. Totowa, N.J. : Humana Press, ©2004 1588291162
Standard no.:10.1385/1592597653.
Table of Contents:
  • Preface
  • Contributors
  • Part I. Communities and Biofilms
  • 1. Isolation and Molecular Characterization of Seawater Bacteria
  • 2. Reverse Sample Genome Probing to Monitor Microbial Communities
  • 3. T-RFLP Analysis: A Rapid Fingerprinting Method for Studying Diversity, Structure, and Dynamics of Microbial Communities
  • 4. Assessing Bacterial DNA Content in Aquatic Systems
  • 5. Multiplexed Identification and Quantification of Analyte DNAs in Environmental Samples Using Microspheres and Flow Cytometry
  • 6. Molecular Characterization of Microbial Communities From Marine Environments
  • 7. Yeasts: Ecology in Northwest Argentina
  • 8. Aeromonads in Environmental Waters
  • 9. Development of a Vital Fluorescent Staining Method for Monitoring Bacterial Transport in Subsurface Ground Water
  • 10. Bench Scale Flow Cell for Nondestructive Imaging of Biofilms
  • Part II. Fermented Milks
  • 11. ¿-Galactosidase Assay in Fermented Soymilk Products
  • 12. Considerations to Avoid the Overestimation of Exopolysaccharides Produced by Lactic Acid Bacteria
  • 13. Determination of Oligosaccharides in Fermented Soymilk Products by High-Performance Liquid Chromatography
  • 14. Evaluation of Minimal Nutritional Requirements of Lactic Acid Bacteria Used in Functional Foods
  • Part III. Nucleic Acids, Recovery, and Determination
  • 15. A Simple Method for Obtaining DNA Suitable for RAPD Analysis From Azospirillum
  • 16. PCR Fingerprinting of rRNA Intergenic Spacer Regions for Molecular Characterization of Environmental Bacteria Isolates
  • 17. A DNA-DNA Hybridization Method for the Detection and Quantification of Specific Bacterial Taxa in Natural Environments
  • 18. 13 C and 1 H NMR Study of the Glycogen Futile Cycle in Fibrobacter succinogenes
  • 19. Chemical Analysis and Biological Removal of Wood Lipids Forming Pitch Deposits in Paper Pulp Manufacturing
  • 20. Propolis: Chemical Micro-Heterogeneity and Bioactivity
  • 21. Enzymatic Saccharification of Cellulosic Materials
  • 22. Molecular Identification of Microbial Populations in Petroleum-Contaminated Groundwater
  • 23. Identification of Copper-Resistant Microorganisms by PCR
  • 24. Selection of Tolerant Heavy Metal Yeasts From Different Polluted Sites
  • 25. Medium for Differential Enumeration of Lactobacillus casei and Lactobacillus acidophilus From Lyophilized Mixed Cultures
  • 26. Silk as a Means of Recovering Bacteriocin From a Culture Medium
  • 27. Optimization of a Method for Isolating Plasmid DNA From Lactic Acid Bacteria From Wine
  • 28. Simultaneous and Sequential Methods to Study Interactions Between Yeast and Lactic Acid Bacteria From Wine
  • 29. Method for Determining Lindane Concentration in Water and Solid Samples
  • 30. Extracellular Hydrolytic Enzymes Produced by Yeasts
  • 31. Extracellular Hydrolytic Enzymes Produced by Phytopathogenic Fungi
  • 32. An Analysis of Microorganisms in Environments Using Denaturing Gradient Gel Electrophoresis
  • 33. Testing for Evolutionary Correlations in Microbiology Using Phylogenetic Generalized Least Squares
  • 34. Conjugated Linoleic Acid Detection Produced by Dairy Starter Cultures
  • Part IV. Reviews
  • 35. Techniques for Manipulating the Bacterial Endophyte Bacillus mojavensis
  • 36. Engineering of Bacteria Using the Vitreoscilla Hemoglobin Gene to Enhance Bioremediation of Aromatic Compounds
  • 37. The Use of Chemical Shift Regents and 23 Na NMR to Study Sodium Gradients in Microorganisms
  • 38. Denaturing Gradient Gel Electrophoresis (DGGE) as a Fingerprinting Tool for Analyzing Microbial Communities in Contaminated Environments
  • Index