Genetic recombination : reviews and protocols /
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Imprint: | Totowa, N.J. : Humana Press, ©2004. |
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Description: | 1 online resource (x, 260 pages) : illustrations (some color) |
Language: | English |
Series: | Methods in molecular biology, 1064-3745 ; v. 262 Methods in molecular biology (Clifton, N.J.) ; v. 262. |
Subject: | |
Format: | E-Resource Book |
URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11129225 |
Table of Contents:
- Preface
- Contributors
- Part I. Studying Recombination Events in Eukaryotes
- 1. Determination of Mitotic Recombination Rates by Fluctuation Analysis in Saccharomyces cerevisiae
- 2. Determination of Intrachromosomal Recombination Rates in Cultured Mammalian Cells
- 3. Intrachromosomal Homologous Recombination in Arabidopsis thaliana
- 4. Analysis of Recombinational Repair of DNA Double-Strand Breaks in Mammalian Cells With I-Scel Nuclease
- 5. Transformation of Monomorphic and Pleomorphic Trypanosoma brucei
- 6. Forward Genetic Screens for Meiotic and Mitotic Recombination-Defective Mutants in Mice
- Part II. Recombination as a Reporter of Genomic Instability
- 7. Detecting Carcinogens With the Yeast DEL Assay
- 8. In Vivo DNA Deletion Assay to Detect Environmental and Genetic Predisposition to Cancer
- Part III. Recombination as a Tool for Producing Targeted Genetic Modification
- 9. Gene Targeting at the Chromosomal Immunoglobulin Locus: A Model System for the Study of Mammalian Homologous Recombination Mechanisms
- 10. DNA Fragment Transplacement in Saccharomyces cerevisiae: Some Genetic Considerations
- 11. Targeted Gene Modification Using Triplex-Forming Oligonucleotides
- 12. Using Nucleases to Stimulate Homologous Recombination
- 13. Enhancement of In Vivo Targeted Nucleotide Exchange by Nonspecific Carrier DNA
- Part IV. Biochemistry of Recombination
- 14. Chromatin Immunoprecipitation to Investigate Protein-DNA Interactions During Genetic Recombination
- 15. Holliday Junction Branch Migration and Resolution Assays
- Index