Genetic recombination : reviews and protocols /

Genetic recombination : reviews and protocols /

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Bibliographic Details
Imprint:	Totowa, N.J. : Humana Press, ©2004.
Description:	1 online resource (x, 260 pages) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; v. 262 Methods in molecular biology (Clifton, N.J.) ; v. 262.
Subject:	Genetic recombination. Recombination, Genetic. Gene Expression. Gene Targeting. Genetic Engineering. Genetic recombination.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11129225

Hidden Bibliographic Details
Other authors / contributors:	Waldman, Alan S.
ISBN:	1588292363 9781588292360 1592597610 9781592597611 1280358289 9781280358289 9786610358281 6610358281
Notes:	Includes bibliographical references and index. English. Print version record.
Summary:	Genetic recombination plays an essential role in such vitally important biological processes as DNA repair, regulation of gene expression, disease etiology, meiotic chromosome segregation, and evolution. In Genetic Recombination: Reviews and Protocols, prominent international investigators actively engaged in recombination research describe in great detail their best techniques for studying recombination. The methods range from approaches and model systems to be used in a variety of eukaryotic organisms and in a mammalian parasite, to biochemical analyses useful for furthering the understanding of recombination mechanisms. Additional techniques use recombination as a reporter of genomic instability in lower and higher eukaryotes and as a tool for producing targeted genetic modification. Each readily reproducible method includes step-by-step instructions, a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Genetic Recombination: Reviews and Protocols offers both novice and experienced investigators an illuminating synthesis of what we currently know about recombination, as well as a wide-ranging collection of powerful tools for elucidating its mechanisms and regulation throughout biology today.
Other form:	Genetic recombination
Standard no.:	10.1385/1592597610.

Description
Summary:	Genetic recombination, in the broadest sense, can be defined as any process in which DNA sequences interact and undergo a transfer of information, producing new "recombinant" sequences that contain information from each of the original molecules. All organisms have the ability to carry out recombination, and this striking universality speaks to the essential role recombination plays in a variety of biological processes fundamentally important to the maintenance of life. Such processes include DNA repair, regulation of gene expression, disease etiology, meiotic chromosome segregation, and evolution. One important aspect of recombination is that it typically occurs only between sequences that display a high degree of sequence identity. The stringent requirement for homology helps to ensure that, under normal circumstances, a cell is protected from deleterious rearrangements since a swap of genetic information between two nearly identical sequences is not expected to dramatically alter a genome. Recombination between dissimilar sequences, which does happen on occasion, may have such harmful consequences as chromosomal translocations, deletions, or inversions. For many organisms, it is also important that recombination rates are not too high lest the genome become destabilized. Curiously, certain organisms, such as the trypanosome parasite, actually use a high rate of recombination at a particular locus in order to switch antigen expression continually and evade the host immune system effectively.
Physical Description:	1 online resource (x, 260 pages) : illustrations (some color)
Bibliography:	Includes bibliographical references and index.
ISBN:	1588292363 9781588292360 1592597610 9781592597611 1280358289 9781280358289 9786610358281 6610358281
ISSN:	1064-3745 ;