N6-methyladenosine-dependent rna structural switches modulate RNA-protein interactions /
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| Author / Creator: | Liu, Nian, author. |
|---|---|
| Imprint: | 2015. Ann Arbor : ProQuest Dissertations & Theses, 2015 |
| Description: | 1 electronic resource (122 pages) |
| Language: | English |
| Format: | E-Resource Dissertations |
| Local Note: | School code: 0330 |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/10773389 |
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| 100 | 1 | |a Liu, Nian, |e author. | |
| 245 | 1 | 0 | |a N6-methyladenosine-dependent rna structural switches modulate RNA-protein interactions / |c Liu, Nian. |
| 260 | |c 2015. | ||
| 264 | 1 | |a Ann Arbor : |b ProQuest Dissertations & Theses, |c 2015 | |
| 300 | |a 1 electronic resource (122 pages) | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a computer |b c |2 rdamedia | ||
| 338 | |a online resource |b cr |2 rdacarrier | ||
| 500 | |a Advisors: Tao Pan Committee members: Chuan He; Joseph Piccirill. | ||
| 502 | |b Ph.D. |c The University of Chicago, Division of the Physical Sciences, Department of Chemistry |d 2015. | ||
| 506 | |a This item is not available from ProQuest Dissertations & Theses. | ||
| 510 | 4 | |a Dissertation Abstracts International, |c Volume: 77-05(E), Section: B. | |
| 520 | |a Various chemical modifications have been found in cellular RNAs, but their functions remain an uncharted territory. N6 -methyladenosine (m6A), the most abundant and dynamic internal modification in eukaryotic messenger RNA (mRNAs), is indispensable for cell viability, pluripotency and human health, but how m6A achieves such wide-ranging biological functions remains unclear. The m 6A functional studies have been hindered by the lack of methods for its precise detection. To address this problem, I developed one method to detect m6A modification status at nucleotide-resolution, and identified exact m6A sites in human mRNAs and long non-coding RNAs (lncRNAs). Afterwards, I discovered that m6A alters the local RNA structure to control the RNA-structure-dependent accessibility of RNA binding sites, thus affecting RNA-protein interactions; I termed this mechanism 'm6A-switch'. Two members of heterogeneous nuclear ribonucleoproteins (HNRNPs), HNRNPC and HNRNPG, are found to be regulated by m6A-switches. These m6A-switch-regulated HNRNPC/G binding activities affect the RNA abundance and alternative splicing events. These findings illustrate how RNA-binding proteins gain regulated access to their RNA binding sites through m6A-dependent RNA structural remodeling, and provides a new direction for investigating RNA-modification-coded cellular biology. | ||
| 546 | |a English | ||
| 590 | |a School code: 0330 | ||
| 690 | |a Molecular biology. | ||
| 690 | |a Biochemistry. | ||
| 690 | |a Biology. | ||
| 710 | 2 | |a University of Chicago. |e degree granting institution. | |
| 720 | 1 | |a Tao Pan |e degree supervisor. | |
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