Imaging and analysis pipelines for long-term high-resolution quantification of the dynamics of individual organism behavior /

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Bibliographic Details
Author / Creator:Wright, Charles Scott, Jr., author.
Ann Arbor : ProQuest Dissertations & Theses, 2015
Description:1 electronic resource (606 pages)
Format: E-Resource Dissertations
Local Note:School code: 0330
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Other authors / contributors:University of Chicago. degree granting institution.
Notes:Advisors: Norbert F. Scherer; David Biron Committee members: Aaron R. Dinner; Michael Glotzer; Michael Rust.
Dissertation Abstracts International, Volume: 77-02(E), Section: B.
Summary:I have developed pipelines for the experimental acquisition and computational analysis of long-term high-resolution images of large populations of individual organisms. These workflows allow for the quantification of the dynamics of behavior in a statistically meaningful way. I first discuss an experimental apparatus I developed to obtain data of neuronal activity in freely behaving nematodes, then highlight the software that I wrote to analyze this data, and which I subsequently adapted for a different experimental setup for measuring calcium transients. I then review two alternative approaches to analyzing worm behavior. Next, I switch perspectives and detail my work to develop a novel approach to obtain multi-generational optical microscopy measurements of a selected set of single bacterial cells, adding the essential experimental and analytical components to construct an end-to-end pipeline from dynamic single-cell imaging experiments to automated image and data analysis. Finally, I present two manuscripts that utilized this system to garner novel theoretical insights into bacterial growth and division.