Epithelial rotation and planar polarity during tissue elongation in Drosophila.

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Bibliographic Details
Author / Creator:Cetera, Maureen.
Description:165 p.
Format: E-Resource Dissertations
Local Note:School code: 0330.
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/10168491
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Other authors / contributors:University of Chicago.
Notes:Advisor: Sally Horne-Badovinac.
Thesis (Ph.D.)--The University of Chicago, Division of the Biological Sciences, and The Pritzker School of Medicine, Committee on Developmental Biology, 2015.
Dissertation Abstracts International, Volume: 76-08(E), Section: B.
Summary:Tissue morphogenesis is a dynamic process that requires the orchestrated action of individual cells within the developing unit. The Drosophila egg chamber is an organ-like structure that elongates to form an elliptical egg. During elongation the follicular epithelial cells undergo a collective migration that causes the egg chamber to rotate within its surrounding basement membrane. Rotation coincides with the formation of a "molecular corset", in which actin bundles at the basal surface of the follicle cells and fibrils within the basement membrane are all aligned perpendicular to the elongation axis. The majority of my work has focused on determining the role that rotation plays in building the actin-based component of the corset. Rotation begins shortly after egg chamber formation and requires lamellipodial protrusions at each follicle cell's leading edge. During early stages, rotation is required to maintain the tissue-level actin bundle alignment inherited from the follicle cell precursors, but it becomes dispensable after the basement membrane is polarized. Additionally, I will describe unpublished data that show rotation can play an active role in globally aligning the actin bundles as tissue-level actin alignment is restored after rotation is delayed.
Additional chapters include a methods section describing egg chamber dissection and live imaging techniques along with unpublished data investigating the role of the receptor tyrosine phosphatase Lar and the BM components during late rotation stages. I will also review the current literature in the field and highlight how collective cell migration can be utilized to polarize a tissue for morphogenesis.